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目的 克隆和测定国人血管生成素 1(Ang 1)基因序列。方法 巢式多聚酶链反应(PCR)从婴儿肺组织扩增中国人Ang lcDNA ,克隆入载体 pGEM Teasy ,测定核苷酸序列 ,分析测序结果。结果 通过巢式PCR获得Ang 1cDNA ,和GeneBank的Ang 1序列 (U83 5 0 8)对照 ,两者成熟区cDNA的核苷酸和氨基酸同源性分别大于 99%和大于 98% ;在 3 3 0bp～ 93 0bp间核苷酸同源性为 98% ,氨基酸同源性为 95 %。结论 获得克隆的中国人Ang 1基因成熟区 ,其卷曲卷曲结构域内可能存在一个内部高变区。
Objective To clone and determine the angiopoietin 1 (Ang 1) gene sequence in Chinese. Methods Nested polymerase chain reaction (PCR) was used to amplify Ang l cDNA of Chinese from infant lung tissue and cloned into vector pGEM Teasy. The nucleotide sequence was determined and the sequencing results were analyzed. Results The Ang 1 cDNA was obtained by nested PCR and the Ang 1 sequence (U83 588) of GeneBank. The nucleotide and amino acid homology of mature cDNAs was greater than 99% and greater than 98%, respectively. At 330 bp ~ 93bp nucleotide homology was 98%, amino acid homology was 95%. Conclusion There may be an internal hypervariable region in the mature region of Ang 1 gene of cloned Chinese.