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人们日益重视对生物学和医学方面氧毒与自由基效应的设想,即细胞损伤是由自由基增多造成。Aitken等指出人精子可产生活性氧基,并已证实某种情况精子功能低下的男性不育与活性氧自由基(O_2(?))过高有关。现对一种甲基黄嘌呤衍生物,即己酮可可碱(Pentoxifyllin)是否抑制人精子超氧化物阴离子如O_2(?)的产生进行体外实验研究。研究采用117份不育精液标本,室温液化后用WHO标准法行精子数量、活率、活力、形态及低渗肿胀实验(HOS)。这些指标与超氧化物阴离子的测定均在收集标本后2小时内完成。由于超氧化物阴离子不仅由精子而且也由中性白细胞产生,
Increasing emphasis on biological and medical oxygen and free radical effects of the idea that cell damage is caused by an increase in free radicals. Aitken et al. Pointed out that human sperm can produce reactive oxygen species and it has been demonstrated that male infertility with sperm dysfunction is associated with an excess of reactive oxygen species (O 2 (?)) In some cases. An in vitro experimental study was conducted on whether a methylxanthine derivative, Pentoxifyllin, inhibits the production of human sperm superoxide anion such as O 2 (?). Totally, 117 samples of infertile semen were collected. The number of sperm, viability, viability, morphology and hypotonic swelling test (HOS) were determined by WHO standard method after liquefaction at room temperature. These indicators and determination of superoxide anion are completed within 2 hours after collecting the specimens. Since superoxide anion is produced not only by sperm but also by neutrophils,