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目的研究全缘橐吾根部的化学成分及生物活性。方法采用柱层析对全缘橐吾根部的提取物进行分离提纯得到单体化合物,并用现代波谱方法(核磁共振氢谱、核磁共振碳谱、质谱)确定其化合物结构;用四甲基偶氮唑盐比色法对分离得到的化合物进行抗肿瘤活性评价。结果从全缘橐吾根部提取物中分离得到13种化合物,经结构鉴定分别为:蜂斗菜内酯A(1);8β-Hydroxyeremophil-7(11)-ene-12,8α(4β,6α)-diolide(2);8β-Hydroxyeremophil-7(11)-ene-12,8β(4β,6α-diolide(3);8β-Methoxyeremophil-7(11)-ene-12,8α(4β,6α)-diolide(4);8β-Hydroeremophil-7(11)-ene-12,8α(14β,6α)-diolide(5);异蜂斗菜素(6);蜂斗菜素(7);β-谷甾醇(8);豆甾醇(9);2α-羟基乌苏酸(10);齐墩果酸(11);正棕榈酸(12);对羟基苯甲醛(13)。其中倍半萜类化合物1、2、4、5、6对人肝癌Hep G2细胞、人食管癌Eca109细胞均表现出较强的体外抗肿瘤活性。结论 13种化合物均为首次从该植物中分离得到;体外抗肿瘤活性较强的化合物1、2、4、5、6有望成为天然抗肿瘤先导化合物。
Objective To study the chemical constituents and bioactivities of Allium mongolicum roots. Methods Column chromatography was used to separate and purify the extracts from the root of Radix Rehmanniae. The structures of the compounds were determined by modern spectroscopic methods (1H NMR, 13C NMR, MS) Azolium salt colorimetric method was used to evaluate the antitumor activity of the isolated compounds. Results Thirteen compounds were isolated from the root extracts of Allium angustifolia, and their structures were identified as follows: bebuterol A (1); 8β-Hydroxyeremophil-7 (11) -ene-12,8α dihydroxyyeremophil-7 (11) -ene-12,8β (4β, 6α-diolide (3); 8β-Methoxyeremophil-7 (11) -ene-12,8α (4β, 6α) beta-Hydroeremophil-7 (11) -ene-12,8α (14β, 6α) -diolide (5); Astrohexin (6) Sitosterol (8); stigmasterol (9); 2α-hydroxy ursolic acid (10); oleanolic acid (11); n-palmitic acid Compounds 1, 2, 4, 5 and 6 showed strong antitumor activity in vitro against human hepatocellular carcinoma Hep G2 cells and human esophageal cancer cell line Eca109. CONCLUSION Thirteen compounds were isolated from this plant for the first time. The more active compounds 1, 2, 4, 5, 6 are expected to become natural anti-tumor lead compounds.