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目的:为在体研究切应力改变诱导内皮细胞组织因子的表达建立一种可控性的大鼠颈总动脉狭窄模型。方法:54只SD大鼠随机分为9组,用自制硅胶管套扎左颈总动脉中段,术后不同时相点用TS 420型多普勒血流仪测定血流量,灌注前用DIGITEXα数字血管减影操作系统测定血管内径,用Poiseiulle流体力学公式Tw=32ηQ/πD3计算切应力。结果:颈总动脉狭窄85%后,平均血流量减少了65%,局部切应力发生了显著变化(p<0.01),在7d内无明显改变。结论:硅胶管套扎大鼠颈总动脉中段的狭窄模型,是比较理想的在体检测血流壁切应力改变对血管内皮细胞形态和功能影响的方法。
OBJECTIVE: To establish a controlled rat model of common carotid artery stenosis in order to study the effect of stress-induced alteration on the expression of tissue factor in endothelial cells. Methods: Fifty-four SD rats were randomly divided into 9 groups. The left middle common carotid artery was ligated with homemade silicone tube. The blood flow was measured by TS 420 Doppler flowmeter at different time points after operation. The vessel size was determined by the angiography operating system, and shear stress was calculated using the Poiseiulle equation of fluid Tw = 32ηQ / πD3. Results: After 85% of common carotid artery stenosis, the average blood flow decreased by 65%, and the local shear stress changed significantly (p <0.01), but there was no obvious change within 7 days. Conclusion: The stenosis model of rat common carotid artery ligated with silicone tube is an ideal method to detect the influence of shear stress changes on the morphology and function of vascular endothelial cells.