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目的 建立快速、特异、有效的鉴别甲、乙型流感病毒的方法。方法 根据编码甲、乙型流感病毒膜蛋白M基因的核苷酸序列设计两对引物 ,将这两对引物用于同一逆转录 聚合酶链反应(RT PCR) ,甲、乙型毒株的扩增产物分别为 5 0 6bp和 2 40bp ,根据这些产物在 1.2 %的琼脂糖凝胶电泳上的大小 ,即可鉴别所测毒株的型别。结果 用这一方法对我国 2 4株甲型和 5株乙型流感病毒分离株进行型别鉴定 ,分型结果与血凝抑制试验和核苷酸测定结果完全相符。结论 用上述根据M基因序列设计的RT PCR方法鉴别甲、乙型流感病毒具有快速、简便、特异等特点 ,适用于流感病毒型别鉴定 ,可为临床诊断和及时制定防治措施提供依据
Objective To establish a rapid, specific and effective method for the identification of influenza A and B viruses. Methods Two pairs of primers were designed according to the nucleotide sequences of the M genes encoding the influenza A and B virus membrane proteins. The two pairs of primers were used for the same reverse transcription polymerase chain reaction (RT PCR) The products were 50 6bp and 240bp, respectively. According to the size of these products on 1.2% agarose gel electrophoresis, the type of the tested strains can be identified. Results Using this method, 24 strains of type A and 5 strains of influenza B virus were identified in our country. The typing results were in good agreement with the results of hemagglutination inhibition and nucleotide determination. Conclusion The identification of influenza A and B viruses by using the above-mentioned RT PCR method designed according to the M gene sequence is rapid, simple and specific, and is suitable for the identification of influenza virus types, and can provide a basis for clinical diagnosis and timely prevention and control measures