研究探讨了不同的基因型、幼胚取材时期、4℃处理时间、盾片接种方式、分化及生根条件等对小麦幼胚培养再生成株特性的影响 ,并在此基础上建立了一套高效、可靠、重复性好的小麦组培再生系统。优化条件下 ,该系统从幼胚诱导致密愈伤组织的频率为89% ,致密愈伤组织的分化频率诱导 2周时为 95 % ,培养近 3个月时仍可达 5 0 %以上。此外还发现部分叶状结构当转至新鲜的分化培养基上时能够进一步发育成为芽苗。分化的芽苗在生根培养基上大多生成丛生苗。从基部切开后 ,每棵芽苗 /分蘖均可独立成株。组培苗均可正常地开花结实 The effects of different genotypes, the time of embryogenesis, the treatment time of 4 ℃, the inoculation method of scutellum, the differentiation and rooting conditions on the characteristics of wheat seedling were studied. Based on this, a set of high efficiency , Reliable and reproducible wheat tissue regeneration system. Under optimal conditions, the frequency of the callus induced from immature embryos was 89%, the differentiation frequency of the dense callus was 95% after 2 weeks of induction, and up to more than 50% when cultured for nearly 3 months. In addition, some leafy structures were found to develop into shoots when transferred to fresh differentiation medium. Differentiated shoots mostly produce shoots on rooting medium. After cutting from the base, each shoots / tillers can be independent into a single plant. Tissue culture seedlings can normally flowering and fruiting