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采用随机扩增多态DNA(RAPD)技术对取自六安、龙感湖、淮南和高淳等4个地理群体的日本沼虾(Macrobrachium nipponense)各30个个体进行遗传多样性分析。在事先优化的反应条件下,所使用的OPP、OPX2个系列40个随机引物中,有16个引物扩增出清晰稳定的片断,共产生173个位点,大小在250～2 000 bp,利用PopGen 1.32进行分析,日本沼虾多态位点为75个(多态性片断的比例为43.4%),Shannon信息指数为0.1771～0.2466。遗传距离显示日本沼虾群体间有一定遗传分化,其中高淳群体和龙感湖群体之间遗传距离最大(0.1712),六安群体和淮南群体之间最小(0.1146)。用UPGMA对4个地理群体进行聚类分析,六安和淮南群体首先聚在一起,高淳和龙感湖群体最后。
Random amplified polymorphic DNA (RAPD) was used to analyze the genetic diversity of 30 individuals from 4 geographical populations of Macrobrachium nipponense from Lu’an, Longjianhu, Huainan and Gaochun. Under the pre-optimized reaction conditions, out of 40 random primers of OPP and OPX2 series used, 16 primers amplified clear and stable fragments with a total of 173 loci ranging in size from 250 to 2 000 bp PopGen 1.32 analysis showed that there were 75 polymorphic loci in Macrobrachium nipponense (the percentage of polymorphic fragments was 43.4%), Shannon’s information index was 0.1771 ~ 0.2466. The genetic distance indicated that there was a certain genetic differentiation among the Japanese marsh shrimp populations. The genetic distance between Gaochun and Longjuehu was the largest (0.1712) and the lowest (0.1146) between Lu’an and Huainan populations. UPGMA clustering analysis of four geographical groups, Liu’an and Huainan groups first together, Gaochun and Dragon Lake last.