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背景:近年研究表明,黄芪和当归具有抗自由基的作用,对肾缺血再灌注损伤具有保护作用。目的:观察黄芪、当归注射液在肾缺血再灌注损伤中对腺苷三磷酸酶(ATP酶)的保护作用及机制。设计:以实验动物为研究对象的观察对比实验。单位:一所医学院的生理教研室及肾功能保护研究室。材料:本实验于2001-01/2001-03在泸州医学院生理实验室完成。选择健康成年日本大耳白兔33只,由泸州医学院实验动物中心提供,雌雄不拘,体质量(1.63±0.22)kg。按随机数字表法分为假手术对照组8只、单纯缺血再灌注组8只、黄芪注射液+缺血再灌注组(黄芪组)8只、当归注射液+缺血再灌注组(当归组)9只。方法:术前1d、手术当天、术后1d,黄芪组、当归组每天分别静脉注射药物(黄芪1.25g/kg、当归12.5g/kg),对照组和单纯缺血再灌注组每天注射生理盐水5mL/kg。肾缺血1h再灌注48h后,下腔静脉采血,取右肾上极组织置入30mL/L戊二醛固定,下极组织制成组织匀浆。电镜检查肾组织超微结构,测血清肌酐含量及肾组织中ATP酶活性。主要观察指标:肾组织超微结构、血清肌酐含量及肾组织中ATP酶活性。结果:单纯缺血再灌注组肾组织变性显著,黄芪组、当归组病变较单纯缺血再灌注组明显减轻。单纯缺血再灌注组血清肌酐含量明显高于假手术对照组(P<0.05);黄芪组、?
Background: Recent studies have shown that Astragalus membranaceus and Angelica sinensis have anti-free radical effects and have protective effects on renal ischemia-reperfusion injury. Objective: To observe the protective effect of astragalus and angelica injection on adenosine triphosphatase (ATPase) in renal ischemia reperfusion injury and its mechanism. Design: Observed and contrasted experiments with experimental animals. Unit: A Department of Physiology and a Laboratory of Renal Function Protection at a medical school. MATERIALS: This experiment was performed at the Physiology Laboratory of Luzhou Medical College from January 2001 to March 2001. Thirty-three healthy adult Japanese white rabbits were selected and provided by the Experimental Animal Center of Luzhou Medical College, with either male or female, body mass (1.63±0.22) kg. According to the random number table method, 8 were divided into sham-operated control group, 8 cases of simple ischemia-reperfusion group, 8 cases of Huangqi injection+ischemia reperfusion group (Huangqi group), 8 cases of Angelica injection+ischemia-reperfusion group (Danggui Group) 9 only. Methods: 1 day before operation, 1 day after surgery, and 1 day after surgery, each of the Astragalus and Angelica groups received intravenous injections of astragalus (1.25g/kg, angelica 12.5g/kg), and the control group and the ischemic reperfusion group received daily saline injections. 5mL/kg. After 48 hours of 1 hour reperfusion of renal ischemia, blood was collected from the inferior vena cava. The right kidney upper pole tissue was placed in 30 mL/L of glutaraldehyde, and the lower pole tissue was made into tissue homogenate. The ultrastructure of renal tissue was examined by electron microscopy. Serum creatinine content and ATPase activity in kidney tissue were measured. MAIN OUTCOME MEASURES: Renal tissue ultrastructure, serum creatinine content, and ATPase activity in kidney tissue. Results: The renal tissue degeneration was significant in the ischemic reperfusion group. The lesions in the Huangqi group and the Chinese angelica group were significantly less than those in the ischemia-reperfusion group. The serum creatinine level in the ischemia-reperfusion group was significantly higher than that in the sham-operated control group (P<0.05);