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用细胞培养、免疫细胞化学染色、图像分析及流式细胞术观察低氧和猪肺动脉缺氧内皮细胞条件培养液(HECCM)对体外培养的新生大鼠肺血管周细胞 (PC)α- SM- actin、 CD34 、 S- 10 0和 PCNA的表达及细胞周期的影响。结果发现 ,低氧和 HECCM可促进 PC表达α- SM- actin和 PCNA,而抑制 CD34 和 S- 10 0的合成 ,促进 PC由静止期 (G0 /G1 期 )进入 DNA合成期 (S期 )及有丝分裂期 (G2 +M期 )。提示 ,低氧不仅能促进 PC增殖 ,而且还可促进其向平滑肌样细胞转化。故 PC是慢性低氧性肺动脉高压时无肌型细动脉肌化的重要细胞来源。
Cell culture, immunocytochemical staining, image analysis and flow cytometry were used to observe the effect of hypoxic and porcine pulmonary arterial hypoxic endothelial cell conditioned medium (HECCM) on the expression of α-SM- actin, CD34, S-100 and PCNA expression and cell cycle. The results showed that hypoxia and HECCM promoted the expression of α-SM-actin and PCNA in PC, inhibited the synthesis of CD34 and S-10 0, and promoted the progression of PC from silent phase (G0 / G1 phase) to DNA synthesis phase Mitosis (G2 + M phase). Tip, hypoxia can not only promote the proliferation of PC, but also promote its transformation to smooth muscle-like cells. Therefore, PC is an important cell source for muscle-less muscular arterioles in chronic hypoxic pulmonary hypertension.