Prevalence and genotype distribution of Enterobius vermicularis among kindergarteners in Shiraz and

来源 :亚太热带医药杂志(英文版) | 被引量 : 0次 | 上传用户:kniba98
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Objective: To study the prevalence and genotype of Enterobius (E.) vermicularis from adhesive tape samples in the cities of Shiraz and Khorramabad, Iran. Methods: A total of 1000 adhesive tape samples from kindergartens in Shiraz (500 samples) and Khorramabad (500 samples) were collected and tested using a microscope to find E. vermicularis egg/s. A questionnaire was filled out for each sample. In order to characterize the genotype of E. vermicularis, the PCR-sequencing method of the mitochondrial cytochrome C oxidase subunit 1 (cox1) gene was used. Genomic DNA was extracted from the positive scotch tape samples of E. vermicularis. The cox1 gene was amplified by the polymerase chain reaction and sequenced. The sequence data were aligned using the BioEdit software and compared with the published sequences in GenBank. Phylogenetic analysis was performed using the maximum likelihood method. Results: The parasitological method showed that 15 out of the 500 samples from Shiraz (3.00%) and 12 out of the 500 samples from Khorramabad (2.40%) were infected with E. vermicularis eggs. BLAST analysis indicated that the sequenced isolates belonged to E. vermicularis genotype B while three different haplotypes were also identified. Conclusions: This is the first study on genotyping E. vermicularis in the cities of Shiraz and Khorramabad. Considering the public health importance of the disease, further studies are necessary to characterize the genotype of E. vermicularis in human populations from other regions of Iran.
其他文献
目的:探讨染色体非多态性位点多重荧光定量PCR(QF-PCR)快速产前诊断胎儿非整倍体畸形的可行性。   方法:在非整倍体最常涉及的21,16,18,13号常染色体及X,Y性染色体功能基因区设
Objective: To determine the morphological and molecular characterization of Acanthamoeba isolates from contact lens paraphernalia in Malaysia and to investigate
Objective: To provide preliminary descriptions of the spread, burden and related medical capacity characteristics of the ASEAN countries.Methods: We utilized th
本文通过对荣华二采区10
Objective: To assess levels of HBsAb amongst infants who received hepatitis B vaccine in the Expanded Program on Immunization in Vietnam. Methods: A cross-secti
目的 分析中医综合疗法在肛肠术后尿潴留中的价值.方法 将2017年1月至2019年1月到我院治疗的210例肛肠术后尿潴留患者作为研究对象,采用抽签法将其分为对照组和治疗组.其中对
目的:   比较汉防己甲素、顺铂与汉防己甲素联合顺铂瘤体内注射及腹腔内注射对荷H22实体瘤模型及荷H22腹水瘤模型的肿瘤抑制效果。   方法:   选用成熟雄性ICR小鼠皮
2008年北京奥运会是中国改革的一个新契机。无论是绚丽夺目的开幕式庆典,还是位居第一的51枚奥运金牌;无论是高楼林立的现代化街道,还是北京市民和志愿者的真诚微笑,都给世界
本文研究了雌激素对AD大鼠和去卵巢大鼠学习记忆的影响及作用机制。本研究分为三个部分:   第一部分:AD大鼠模型的建立及Aβ对其行为、病理及tau蛋白磷酸化水平的影响。  
随着现今社会人们生活形式的转变,各种疾病的发病率越来越高,加上人们对健康问题的逐渐重视,医院的人流量越来越大,医院的业务越来越繁杂,因此, 便捷化的医院信息技术管理变