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目的以发光细菌作为毒性测试物种,以毒性较强的3种重金属铊、锑和钡作为研究对象,分析它们对发光细菌的毒性特点。方法将3种有毒重金属铊、锑和钡初步建立毒性测试模型,用生物毒性测试仪测定样品及空白的发光度,计算相对发光率。结果发光菌的相对发光率与毒物的浓度呈负相关,其相关系数为-0.88~-0.99,P<0.05。铊、锑和钡作用15 min时使发光细菌产生50%光损失时的浓度(EC50值)分别为21.60、148.56和1 094.50 mg/L。结论该方法在有毒重金属的毒性监测和评价方面可以弥补理化方法分析毒性时的不足,具有一定的应用前景。
OBJECTIVE To study the toxicity of luminescent bacteria to luminescent bacteria by using luminescent bacteria as toxicological test species and the three highly toxic heavy metals, thallium, antimony and barium, as research objects. Methods Toxicity test models of three toxic heavy metals, thallium, antimony and barium, were initially established. The toxicity of the sample and the blank was measured with a biological toxicity tester to calculate the relative luminescence rate. Results The relative luminescence rate of luminescent bacteria was negatively correlated with the concentration of poisons. The correlation coefficient was -0.88 ~ -0.99, P <0.05. Concentrations (EC50 values) of 50% light loss of luminescent bacteria at 15 min with thallium, antimony and barium were 21.60, 148.56 and 1094.50 mg / L, respectively. Conclusion This method can make up for the shortage of physical and chemical methods in toxicological monitoring and evaluation of toxic heavy metals, and has certain application prospects.