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目的进一步研究ｍｄｒ１基因的表达与原发性肺癌多药耐药的关系。方法应用逆转录多聚酶链式反应（ＲＴ－ＰＣＲ）方法对４３例未治、５例化疗后的原发性肺癌和３３例癌旁正常肺组织ｍｄｒ１基因的表达进行分析。结果４８例癌标本中有１５例被观察到ｍｄｒ１基因的过度表达，主要分布在非小细胞肺癌（ＮＳＣＬＣ）中，仅１例化疗后标本ｍｄｒ１阳性。ｍｄｒ１基因的过度表达与肿瘤大小，淋巴结转移和病期无明显相关性。随访一年后发现，过度表达ｍｄｒ１基因的病例，其复发率高于无过度表达者。结论在未治的原发性肺癌尤其是ＮＳＣＬＣ中可检测到ｍｄｒ１基因的过度表达，并且可能是提示不良预后的指标。
Objective To further investigate the relationship between mdr1 gene expression and multidrug resistance in primary lung cancer. Methods Reverse transcriptase polymerase chain reaction (RT-PCR) method was used to analyze the expression of mdr1 gene in 43 cases of untreated, 5 cases of primary lung cancer after chemotherapy and 33 cases of normal lung tissue adjacent to cancer. Results Overexpression of mdr1 gene was observed in 15 out of 48 cancer specimens, mainly in non-small cell lung cancer (NSCLC). Only 1 case of mdr1 was positive after chemotherapy. The overexpression of mdr1 gene was not associated with tumor size, lymph node metastasis and stage. After one year of follow-up, it was found that cases with over-expressed mdr1 gene had a higher relapse rate than those without overexpression. Conclusion Overexpression of mdr1 gene can be detected in untreated primary lung cancer, especially NSCLC, and it may be an indicator of poor prognosis.