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建立了反相高效液相色谱测定保健品中左旋肉碱含量的方法。采用Nucleosil C18(250mm×4.6mm,5μm)色谱柱,以甲醇-0.05mol.mL-1磷酸二氢钾(用80%磷酸调pH为2.5)(8∶92)为流动相,流速为0.6mL/min,用紫外检测器在210nm波长处检测。标准品及样品均采用0.5mmol/L盐酸溶解,且样品要在30℃下超声提取30min。结果表明,左旋肉碱有良好线性关系(r2=0.9997),样品的分离程度较好,相对标准偏差(RSD)为1.54%,加标回收率为100.08%—117.89%。该方法较为简便,快速,有良好的准确度。可用于肉碱类保健品中左旋肉碱含量的测定。
A RP-HPLC method for the determination of L-carnitine in health products was established. The mobile phase was a Nucleosil C18 (250mm × 4.6mm, 5μm) column with methanol-0.05mol.mL-1 potassium dihydrogen phosphate (adjusted to pH 2.5 with 80% phosphoric acid) (8:92) at a flow rate of 0.6mL / min, with UV detector detection at 210nm wavelength. Standards and samples were dissolved with 0.5mmol / L hydrochloric acid, and the sample to 30 ℃ ultrasonic extraction 30min. The results showed that L-carnitine had a good linear relationship (r2 = 0.9997). The sample was well separated with a relative standard deviation (RSD) of 1.54% and a spiked recoveries of 100.08% -117.89%. The method is relatively simple, fast and has good accuracy. Can be used for carnitine health products L-carnitine determination.