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目的：研究石杉碱甲对基底核大细胞部（ＮＢＭ）损毁诱导的工作记忆障碍的影响．方法：采用八臂迷宫延迟插板的程序研究空间记忆．胆碱乙酰转移酶（ＣｈＡＴ）活力测定采用［３Ｈ］乙酰辅酶Ａ转变成［３Ｈ］乙酰胆碱的方法．结果：单侧损毁ＮＢＭ（卡因酸００２μｍｏｌ）导致空间记忆障碍．在不同的延迟间隔，大鼠完成程序产生的正确数减少和错误数增多．损毁侧大脑皮层ＣｈＡＴ酶的含量下降了大约４０％．石杉碱甲（０２ｍｇ·ｋｇ－１实验前３０ｍｉｎｉｐ）改善这种空间记忆障碍．毒扁豆碱（０２－０３ｍｇ·ｋｇ－１实验前２０ｍｉｎｉｐ）也有改善作用．结论：完整的ＮＢＭ是空间记忆形成的关键．石杉碱甲有效的改善ＮＢＭ损毁导致的空间记忆障碍．
OBJECTIVE: To study the effects of huperzine A on the impairment of working memory induced by basal ganglia (NBM) damage. Methods: Eight-arm labyrinth delay flapper program was used to study spatial memory. Choline Acetyltransferase (ChAT) Activity Assay [3H] Acetyl-CoA is converted to [3H] Acetylcholine. Results: Unilateral damage to NBM (Ca (2+) mol) resulted in spatial memory impairment. At different delay intervals, the correct number of rats completed and the number of errors increased. The content of ChAT enzyme in the damaged side of the cerebral cortex decreased by about 40%. Huperzine A (0 2mg · kg-1 30minip before the experiment) to improve this spatial memory impairment. Physostigmine (0 2-0 3mg · kg-1 20minip before the experiment) also have improved. Conclusion: The complete NBM is the key to the formation of spatial memory. Huperzine A effectively improves spatial memory impairment caused by NBM damage.