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目的:研究玄参及其拆分组分对异丙肾上腺素(ISO)诱发大鼠实验性心室重构的药理作用及机制。方法:采用大鼠背部皮下注射ISO,连续2 d以递减剂量10、5 mg/kg进行,3 mg/kg的剂量维持注射7 d,制备心室重构模型。经药干预21 d后,检测大鼠左心室(LVMI)及全心室质量指数(HMI);酶联免疫法检测血清心房利钠肽(ANP)、内皮素-1(ET-1)及心肌组织血管紧张素Ⅱ(AngⅡ)含量,同时进行心肌组织病理学观察。结果:玄参全成分、小极性环烯醚萜苷组分可改善模型大鼠LVMI、HMI,降低血清ANP、ET-1及心肌组织AgnⅡ含量;多糖组分仅能降低AngⅡ、ET-1的含量。结论:玄参抑制心室重构的药理作用与小极性环烯醚萜组分和多糖组分有关。其作用机制可能与调节过度激活的神经体液因子,抑制ANP、ET-1、AngⅡ的释放有关。
Objective: To investigate the pharmacological effects and mechanisms of Radix Scrophulariae and its fractions on isoproterenol (ISO) -induced experimental ventricular remodeling in rats. Methods: The rats were injected subcutaneously with ISO at the back for 2 days in a decreasing dose of 10,5 mg / kg and the dose of 3 mg / kg was injected for 7 days to prepare a ventricular remodeling model. The left ventricular (LVMI) and whole heart ventricular mass index (HMI) were detected after 21 days of drug intervention. Serum atrial natriuretic peptide (ANP), endothelin-1 (ET- Angiotensin Ⅱ (AngⅡ) content, at the same time myocardial histopathology observation. Results: All the components of Scrophulariaceae and small polar iridoid glycosides can improve LVMI, HMI, decrease serum ANP, ET-1 and myocardial tissue AgnⅡlevels; polysaccharide can only decrease AngⅡ, ET-1 Content. Conclusion: The pharmacological effects of Scrophulariae on ventricular remodeling are related to the small polar iridoids and polysaccharides. Its mechanism may be related to regulating the activation of neurohumoral factors, inhibiting the release of ANP, ET-1 and AngⅡ.