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研究目的探讨蝙蝠葛苏林碱(DSL)对局部脑缺血-再灌注损伤的治疗作用。研究背景已确证某些钙通道阻断剂(如尼莫地平)有抗脑缺血作用。DSL是新近证明的、能选择性作用于神经细胞的钙通道阻断剂,它对脑缺血的影响尚未见研究报道。研究方法 40只wistar雄性大鼠随机分为5组。Ⅰ、Ⅱ、Ⅲ是低、中、高3个不同剂量DSL的实验组;Ⅳ为阴性对照组;Ⅴ为尼莫地干阳性对照组。用尼龙线经颈内动脉可逆性插入到大脑前动脉内,建立大鼠局部脑缺血再灌注模型。脑电图在双侧顶区记录。研究结果 MCA阻断后,缺血侧的EEG波幅降低,甚至成电平线,然后逐渐恢复,1小时后可恢复到50％以上。复通后1小时Ⅱ、Ⅲ及Ⅴ组的EEG波幅有明显增高,与阴性对照的Ⅳ组差异显著(P<0.05),且这3组动物的生存时间也明显延长。结论 DSL对局部脑缺血-再灌注损伤有一定的保护作用。
The purpose of the study was to investigate the therapeutic effect of batsoullin (DSL) on focal cerebral ischemia-reperfusion injury in rats. Background of the study It has been confirmed that certain calcium channel blockers (such as nimodipine) have anti-cerebral ischemia effects. DSL is a newly demonstrated calcium channel blocker that selectively acts on nerve cells. Its effects on cerebral ischemia have not been reported. Methods 40 wistar male rats were randomly divided into 5 groups. I, II, and III are experimental groups of low, medium, and high doses of DSL with different doses; IV is a negative control group; V is a nimodipine positive control group. A rat model of focal cerebral ischemia and reperfusion was established by reversibly inserting the nylon artery into the anterior cerebral artery through the internal carotid artery. Electroencephalograms are recorded in the bilateral apical region. Study Results After MCA blockade, the amplitude of EEG on the ischemic side decreased, even on the leveling line, and then gradually recovered, recovering to more than 50% after 1 hour. The amplitude of EEG in group II, III, and V increased significantly at 1 hour after reconstitution, which was significantly different from that of group IV of negative control (P<0.05), and the survival time of these three groups of animals was also prolonged. Conclusion DSL has a protective effect on focal cerebral ischemia-reperfusion injury.