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对云南部分兰花种植区的文心兰、蝴蝶兰、大花惠兰上发生的病毒病进行了调查与病原鉴定。兰花感染病毒后,主要以在叶片形成黑褐色的坏死斑为主。在文心兰不同品种中,T系列的病毒病发病率最高。经电子显微镜、血清学和RT-PCR等方法综合鉴定,确定危害云南兰花的主要病毒为建兰花叶病毒(Cymbidi-um mosaic virus,CyMV)和齿兰环斑病毒(Odontoglossum ringspot virus,ORSV),其中CyMV为优势种,有时出现CyMV和ORSV复合侵染的情况。
The virus disease in Oncidium, Phalaenopsis, Dahua Whelan in Yunnan part of the orchid planting area was investigated and the pathogen was identified. Orchid infection virus, mainly in the formation of dark brown necrosis of the main leaves. Among the different varieties of Oncidium, the T series has the highest incidence of viral diseases. The main viruses that damage Yunnan orchid were identified as Cymbidium mosaic virus (CyMV) and Odontoglossum ringspot virus (ORSV) by electron microscopy, serology and RT-PCR. Among them, CyMV is the dominant species, and sometimes complex infection with CyMV and ORSV occurs.