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目的分析细胞因子水平、免疫状态以及人疱疹病毒8型(HHV-8)和非洲淋巴细胞瘤病毒(EBV)感染与新疆卡波西肉瘤(KS)发病的关系。方法采用酶联免疫吸附试验(ELISA)检测血清细胞因子水平,HHV-8检测采用套式聚合酶链反应。结果KS患者白细胞介素-6(IL-6)、血管内皮细胞生长因子(VEGF)、肿瘤坏死因子(TNF-α)、新喋呤(neopterin)和β2-微球蛋白(β2-MG)水平均高于对照组。HHV-8感染率在KS组和对照组分别为82.40%(14/17)和20.59%(14/68),两者的差异有显著的统计学意义(χ2=72.15,P<0.001)。结论IL-6、TNF-α、VEGF等细胞因子的血清水平异常可能与KS的发生或维持KS的症状相关;新喋呤和β2-MG水平的增高反映KS患者存在免疫激活状态;证实了HHV-8与KS发病的关系。
Objective To analyze the relationship between the level of cytokines, immune status, the infection of human herpesvirus type 8 (HHV-8) and African lymphocytovirus (EBV) and the incidence of Kaposi’s sarcoma (KS) in Xinjiang. Methods Serum levels of cytokines were detected by enzyme-linked immunosorbent assay (ELISA), nested polymerase chain reaction (PCR) was used to detect HHV-8. Results The levels of IL-6, VEGF, TNF-α, neopterin and β2-microglobulin (β2-MG) All higher than the control group. The infection rate of HHV-8 in KS group and control group were 82.40% (14/17) and 20.59% (14/68), respectively. The difference was statistically significant (χ2 = 72.15, P <0.001). Conclusion The abnormal serum levels of IL-6, TNF-α, VEGF and other cytokines may be related to the occurrence of KS or the maintenance of the symptoms of KS. The increase of neopterin and β2-MG reflects the immune activation in KS patients. -8 and the pathogenesis of KS.