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目的研究参附注射液对新生大鼠缺氧缺血性脑损伤(HIBD)的干预作用。方法建立新生大鼠HIBD模型,把实验分成两部分(1)观察缺氧缺血后3、7、14和28d各组新生大鼠的体重变化以及各组28d内生存率。(2)流式细胞术测量缺氧缺血前2h,缺氧缺血后2h、12h、24h、3d、7d、14d和28d各时间点新生大鼠海马CA1区神经元的凋亡率,每组实验随机分为4组(每组20只)假手术组,生理盐水对照组,参附预处理组,参附治疗组。结果缺氧缺血后3、7、14和28d,生理盐水对照组大鼠体重的增长均明显小于假手术组(7d8.8g±2.1gvs14.0g±2.9g,均P<0.01),且明显小于参附预处理组(7d11.7g±3.3g,P<0.01或P<0.05),缺氧缺血后7、14、28d生理盐水对照组大鼠体重的增长均明显小于参附治疗组(7d10.9g±2.7g,P<0.01或P<0.05)。各组的体重明显小于假手术组。生理盐水对照组存活率为60%(12/20),参附预处理组存活率为90%(18/20),参附治疗组存活率为85%(17/20),生理盐水对照组存活率明显低于其他各组(P<0.05),其余各组存活率差异无统计学意义(均P>0.05)。缺氧缺血后2h生理盐水对照组、参附治疗组、参附预处理组海马神经元凋亡率开始升高,于缺氧缺血后24h凋亡率达到峰值后开始下降,缺氧缺血后14d降到正常。参附治疗组和参附预处理组海马神经元凋亡情况明显好于生理盐水对照组(24h16.0%±4.2%vs11.9%±2.3%vs18.1%,P<0.05或P<0.01)。结论参附注射液减少了新生大鼠缺氧缺血后神经元凋亡的发生,改善了新生大鼠HIBD后的生长发育,提高了其生存率。
Objective To study the intervention effect of Shenfu Injection on hypoxic-ischemic brain damage (HIBD) in neonatal rats. Methods HIBD model was established in neonatal rats. The experiment was divided into two parts: (1) The body weight of neonatal rats in each group were observed at 3, 7, 14 and 28 days after hypoxia-ischemia and the survival rate within 28 days in each group was observed. (2) The apoptosis rate of hippocampal CA1 neurons in neonatal rats at 2h before hypoxia-ischemia, 2h, 12h, 24h, 3d, 7d, 14d and 28d after hypoxia-ischemia were measured by flow cytometry. Group experiments were randomly divided into 4 groups (20 in each group) sham operation group, saline control group, Shenfu pretreatment group, Shenfu treatment group. Results At 3, 7, 14 and 28 days after hypoxia-ischemia, the body weight of rats in control group was significantly lower than that of sham-operated group (7d8.8g ± 2.1g vs 14.0g ± 2.9g, P <0.01) (P <0.01 or P <0.05). The body weight of rats in the saline control group at 7, 14 and 28 days after hypoxia-ischemia were significantly lower than that of the control group (P <0.01 or P < 7d10.9g ± 2.7g, P <0.01 or P <0.05). The weight of each group was significantly less than the sham group. The survival rate of the saline control group was 60% (12/20), the reference group was pretreatment group survival rate was 90% (18/20), the reference group survival rate was 85% (17/20), saline control group The survival rate was significantly lower than the other groups (P <0.05), while the other groups had no significant difference in survival rate (all P> 0.05). The apoptotic rate of hippocampal neurons in the control group, Shenfu Decoction group and Shenfu Decoction group began to increase at 2h after hypoxia and ischemia, and then decreased at 24h after hypoxic-ischemic insult. 14d after blood fell to normal. The apoptosis of hippocampal neurons in the treatment group and the treatment group was significantly better than that in the saline control group (24h16.0% ± 4.2% vs11.9% ± 2.3% vs18.1%, P <0.05 or P <0.01 ). Conclusion Shenfu injection can reduce neuronal apoptosis after hypoxia-ischemia in neonatal rats, improve the growth and development of newborn rats after HIBD, and improve their survival rate.