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目的 检测转基因细胞移植于猪的创面后有无人表皮细胞生长因子（hEGF）表达。 方法 将种植于生物硅膜上的转基因细胞和正常表皮细胞移植于猪的创面，在移植后第1、3、5、8、12天取移植创面组织活检，采用免疫组织化学方法，测定移植后创面有无hEGF表达。 结果 移植转基因表皮细胞的创面，hEGF表达阳性；移植正常表皮细胞的创面，hEGF表达阴性。 结论 转染hEGF质粒的表皮细胞可在创面表达hEGF。
Objective To detect the expression of human epidermal cell growth factor (hEGF) after transplanting the transplanted cells in pigs. Methods Transgenic cells and normal epidermal cells planted on biological silicon membrane were transplanted to the wounds of pigs. The wounds were grafted on the 1st, 3rd, 5th, 8th and 12th days after transplantation. Immunohistochemistry Wounds with or without hEGF expression. Results Transplantation of epidermal cells transplanted on the wound surface, hEGF positive expression; transplanted normal epidermal cells wound, hEGF negative expression. Conclusion Epidermal cells transfected with hEGF plasmid can express hEGF on the wound surface.