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目的比较FasL诱导2种胶质细胞瘤细胞凋亡作用的差异。方法用脂质体将FasL基因转染NIH3T3纤维母细胞,应用免疫组织化学染色法和RT-PCR法检测NIH3T3/FasL细胞目的基因的表达和转录,用Hochest 33342荧光染色法检验共培养体系中凋亡细胞种类,用TUNEL法检测共培养条件下胶质瘤细胞BT325和U251诱导凋亡作用的差异。结果NIH3T3/FasL细胞可有效表达FasL,与NIH3T3细胞(对照组)相比,有显著的诱导BT325和U251细胞凋亡的作用,对BT325胶质瘤细胞诱导凋亡的作用较U251胶质瘤细胞更为显著(P<0.05)。结论转染了FasL基因的NIH3T3细胞对BT325胶质瘤细胞诱导凋亡的作用比U251胶质瘤细胞更为显著。
Objective To compare the apoptosis effects of FasL induced by two glioblastoma cells. Methods FasL gene was transfected into NIH3T3 fibroblasts by lipofectamine. The expression and transcription of NIH3T3 / FasL cells were detected by immunohistochemical staining and RT-PCR. Hochest 33342 staining was used to detect the expression of FasL gene in the co-culture system The difference of apoptosis induced by BT325 and U251 cells under co-culture condition was detected by TUNEL method. Results NIH3T3 / FasL cells could effectively express FasL. Compared with NIH3T3 cells (control group), NIH3T3 / FasL cells could significantly induce the apoptosis of BT325 and U251 cells and induce the apoptosis of BT325 glioma cells compared with U251 glioma cells More significant (P <0.05). Conclusion NIH3T3 cells transfected with FasL gene have more significant effect on inducing apoptosis in BT325 glioma cells than U251 glioma cells.