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本文介绍高效液相色谱检测低氧时大鼠脑组织中腺苷酸，并将ＡＭＰ、ＡＤＰ、ＡＴＰ分别定量。取大鼠脑组织０．５ｇ以０．５ｍｏｌ／Ｌ高氯酸溶液处理后，用美国Ｗａｔｅｒｓ－２４４型高效液相色谱，４４０型紫外检测器，波长２５４ｎｍ进行检测，用国产ＹＷＧＣ１８不锈钢柱，流动相为甲醇：乙腈：ＩＰＲＡ：水＝８０：６０：１４：３４６（ｍｌ，Ｖ／Ｖ）。方法的回收率ＡＭＰ、ＡＤＰ、ＡＴＰ分别为９８．５６％～１００．４８％，９６．３０％～１０３．７％，９７．９５％～９９．５６％；变异系数分别为４．０８％、３．７１％、３．６８％。
This article describes the detection of adenylate in rat brain tissue by high performance liquid chromatography (HPLC), and quantifies AMP, ADP and ATP respectively. 0.5g rat brain tissue was treated with 0.5mol / L perchloric acid solution, and then detected by Waters-244 high performance liquid chromatograph, 440 UV detector and wavelength 254nm, with domestic YWGC18 stainless steel column Phase is methanol: acetonitrile: IPRA: water = 80: 60: 14: 346 (ml, V / V). The recoveries of AMP, ADP and ATP were 98.56% ~ 100.48%, 96.30% ~ 103.7% and 97.95% ~ 99.56%, respectively. The coefficients of variation were 4.08% 3.71%, 3.68%.