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采集山西省１０个煤矿，井下掘进工作面煤样１６份，每一煤样提取物分成两份；一份进行亚硝化，另一份为非亚硝化煤样，分别进行Ａｍｅｓ试验、ＳＯＳ显色试验、双核微核试验、大鼠骨髓细胞染色体畸变分析试验。结果表明：１６份煤样提取物亚硝化后，其中有１０种Ａｍｅｓ试验、ＳＯＳ显色试验均为阳性，从１０种阳性煤尘中，选１到３种进行染色体畸变分析及双核微核试验，其结果与Ａｍｅｓ、ＳＯＳ显色试验一致，阳性率为６２５％。试验中发现，煤样中挥发分的含量高，亚硝化提取物呈较深的棕色液体，均有致突变性，而随着颜色的加深其致突变性增强，提示煤尘中挥发分含量可间接反应出煤样中有机物的含量，挥发分高的煤样有机物所占比例较大，经亚硝化后，生成具有致突变作用的亚硝基化合物量相对高，故可表现出强的致突变性。这一发现，可采纳较为简便的测定挥发分的试验，对于致突变性的研究的受试煤样进行初筛，以节省大量的人力和物力
Sixteen coal samples were collected from 10 coal mines in Shanxi Province and each coal sample was separated into two samples. One was nitrosated and the other was non-nitrosated coal samples. Ames test and SOS were performed respectively Test, dual-core micronucleus test, chromosomal aberration assay of rat bone marrow cells. The results showed that after 16 samples were nitrosated, 10 kinds of Ames test and SOS color test were positive. Chromosome aberration analysis and dual-core micronucleus test The results were consistent with Ames and SOS chromogenic test, the positive rate was 62.5%. It was found that the content of volatile components in coal sample was high and the nitrite extract was dark brown liquid, all of which were mutagenic. However, the mutagenicity increased with the deepening of color, suggesting that the volatile content in coal dust could be indirectly The results showed that the proportion of organic matter in coal samples was high, and the proportion of coal samples with high volatilities was larger. After nitrosation, the amount of nitroso compounds with mutagenicity was relatively high, which showed strong mutagenicity . This finding allows for a simpler assay to determine volatiles and pre-screening of samples tested for mutagenicity to save significant amounts of human and material resources