论文部分内容阅读
目的:分析丹参有效成分在不同部位的分布。方法:采用HPLC,Shim-pack VP-ODS C18色谱柱(4.6 mm×150mm,5μm),乙腈-0.02%磷酸水为流动相进行梯度洗脱,检测波长270,280 nm,流速1.0 mL.min-1。分别测定丹参粗皮部分,去粗皮部分和原药材有效成分的含量。结果:丹参有效成分的分布部位不同,隐丹参酮、丹参酮ⅡA、原儿茶醛、咖啡酸主要存在于粗皮部分,丹参素和丹酚酸B主要分布在去粗皮部分;栽培丹参粗皮中隐丹参酮、丹参酮ⅡA、原儿茶醛、咖啡酸、丹参素和丹酚酸B的含量分别是去粗皮部分含量的1 933.6%,1 554.7%,700%,268.4%,56.6%,61.1%;野生丹参粗皮中隐丹参酮、丹参酮ⅡA、原儿茶醛、咖啡酸、丹参素和丹酚酸B的含量分别是去粗皮部分含量的1 393.0%,1 822.0%,235.7%,150%,62.8%,90.4%;栽培丹参中丹酚酸B、隐丹参酮、丹参酮ⅡA含量较高。结论:丹参不同部位成分的差异证实丹参传统等级划分的科学性,可以指导我们合理地利用丹参资源。
Objective: To analyze the distribution of active components of Salvia miltiorrhiza in different parts. Methods: The mobile phase was eluted with Shim-Pack VP-ODS C18 column (4.6 mm × 150 mm, 5 μm) using acetonitrile-0.02% phosphoric acid as mobile phase. The detection wavelength was 270,280 nm and the flow rate was 1.0 mL · min-1. Respectively determine the part of Salvia miltiorrhiza, to the rough skin part and the original medicinal content of the active ingredient. Results: The distribution of effective components of Salvia miltiorrhiza was different. Cryptotanshinone, tanshinone Ⅱ A, protocatechuic aldehyde and caffeic acid were mainly present in the bark, danshensu and salvianolic acid B were mainly distributed in the bark part. Cryptotanshinone, The contents of tanshinone IIA, protocatechuic aldehyde, caffeic acid, danshensu and salvianolic acid B were 1 933.6%, 1 554.7%, 700%, 268.4%, 56.6% and 61.1% respectively, The contents of cryptotanshinone, tanshinone ⅡA, protocatechuic aldehyde, caffeic acid, danshensu and salvianolic acid B were respectively 1 393.0%, 1822.0%, 235.7%, 150%, 62.8%, 90.4% Salvia miltiorrhiza B, cryptotanshinone and tanshinoneⅡA were higher in cultivated Salvia miltiorrhiza. Conclusion: The differences of the components of Salvia miltiorrhiza in different parts confirm the scientific classification of traditional Salvia miltiorrhiza, which can guide us to make rational use of Salvia resources.