目的:观察酶促牛黄对小鼠的镇静催眠作用和缺氧保护作用。方法:采用常压缺氧法制备小鼠缺氧模型,观察酶促牛黄对缺氧小鼠耗氧量和存活时间的影响。采用光电计数法观察酶促牛黄对小鼠自主活动的影响,利用戊巴比妥钠阈剂量诱导睡眠试验观察酶促牛黄对小鼠的催眠作用。结果:酶促牛黄灌胃给药0.5,1.0,2.0g.kg-1,能明显降低常压缺氧小鼠的耗氧量,延长生存时间。酶促牛黄灌胃给药1.0g.kg-1,能明显减少小鼠自发活动,增加阈剂量戊巴比妥钠引起的小鼠入睡例数。结论:酶促牛黄对小鼠具有镇静催眠和缺氧保护作用。 OBJECTIVE: To observe the sedative and hypnotic effects and hypoxic protective effect of enzymatic bezoar on mice. Methods: Hypoxia model was established by hypobaric hypoxia. The effects of enzymatic calculus on oxygen consumption and survival time in hypoxic mice were observed. The effect of enzymatic calculus on autonomic activity in mice was observed by photoelectric counting method. The hypnotic effect of enzymatic calculus on mice was observed by using pentobarbital sodium threshold dose-induced sleep test. Results: Enzyme-fed bengal orally administered 0.5,1.0,2.0g.kg-1, can significantly reduce oxygen consumption in hypoxic mice and prolong survival time. Enzyme-fed bengal gavage 1.0g.kg-1, can significantly reduce the spontaneous activity of mice, increasing the threshold dose of pentobarbital sodium-induced mice to fall asleep. Conclusion: Enzyme Niuhuang has sedative, hypnotic and hypoxic protective effects on mice.