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通过简单的高温热解柠檬酸方法制备具有荧光性质的石墨烯,实验发现以辣根过氧化物酶作为催化剂催化焦性没食子酸发生反应为前提,利用石墨烯荧光特性的变化,从而对焦性没食子酸进行定量测定。在pH 7.8的磷酸盐缓冲溶液中,焦性没食子酸在催化酶的作用下发生反应生成有色物质醌,该物质可以迅速使石墨烯的荧光强度发生猝灭现象。据此设计焦性没食子酸荧光猝灭检测法。实验发现随着焦性没食子酸量的不断加入,荧光猝灭现象逐渐增大,并呈现良好的线性。本实验利用新型纳米材料结合荧光法实现了对水样中焦性没食子酸简便、快速地检测。
The graphene with fluorescence properties was prepared by a simple pyrolysis of citric acid. The experiment found that the reaction of pyrogallol by horseradish peroxidase (CAT) as a catalyst catalyzed the change of the fluorescence characteristics of graphene, Acid quantitative determination. In phosphate buffer solution of pH7.8, pyrogallol reacted under the action of catalytic enzyme to generate colored matter quinone, which can quickly quench the fluorescence intensity of graphene. According to the design of pyrogallol fluorescence quenching detection method. The experiment found that with the constant addition of pyrogallic acid, the phenomenon of fluorescence quenching gradually increased and presented a good linearity. In this experiment, the novel nanomaterials combined with fluorescence method to achieve the water sample of the focus of gallic acid simple and rapid detection.