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目的探讨血红蛋白氧合酶/一氧化碳(HO-CO)系统与足月小于胎龄儿(SGA)发病的关系及其机制。方法2004年11月-2005年11月正常分娩的足月SGA(非匀称型SGA)50例。其中母亲无妊娠高血压综合征(PIH)为SGA1组(30例),母亲患中重度PIH为SGA2组(20例),健康足月适于胎龄儿(AGA)20例为对照(AGA组)。新生儿娩出后立即采集脐动脉血标本,用双波长分光光度法测定血红蛋白氧合酶-1(HO-1)的活性,用Chalmers血红蛋白结合及连二亚硫酸钠还原法测定血浆碳氧血红蛋白(HbCO)质量分数。单因素方差分析进行多重比较,直线回归法进行相关分析。结果SGA1组及SGA2组脐血HO-1活性、HbCO质量分数均显著低于AGA组(P<0.01)。SGA1组与SGA2组脐血HO-1活性、HbCO水平比较无显著性差异(Pa>0.05)。AGA组、SGA1组及SGA2组脐血HO-1活性与新生儿出生体质量均呈正相关(Pa<0.05)。结论SGA脐血HO-1活性显著低于ASA,AGA和SGA脐血HO-1活性水平与出生体质量均呈正相关,说明脐血HO-1活性与胎儿宫内发育及新生儿营养状况有关。
Objective To investigate the relationship between the hemoglobin oxygenase / carbon monoxide (HO-CO) system and the incidence of term mild to gestational age (SGA) in children and its mechanism. Methods Fifty cases of full-term SGA (nonsymmetric SGA) under normal delivery were performed from November 2004 to November 2005. The mothers with no pregnancy-induced hypertension (PIH) were in the SGA1 group (n = 30), the mother with moderate or severe PIH was in the SGA2 group (n = 20), healthy full-term suitable for gestational age ). Umbilical artery blood samples were collected immediately after delivery and the activity of hemoglobin oxygenase-1 (HO-1) was measured by dual-wavelength spectrophotometry. The plasma carboxyhemoglobin (HbCO) was measured by Chalmers hemoglobin binding and sodium dithionite reduction. Quality Score. Single factor analysis of variance multiple comparisons, linear regression analysis. Results The activity of HO-1 and the content of HbCO in cord blood of SGA1 group and SGA2 group were significantly lower than those of AGA group (P <0.01). There were no significant differences in HO-1 activity and HbCO level between SGA1 group and SGA2 group (Pa> 0.05). HO-1 activity in cord blood of AGA, SGA1 and SGA2 groups was positively correlated with newborn birth weight (P <0.05). Conclusions HO-1 activity in cord blood of SGA is significantly lower than that in ASA. HO-1 activity in cord blood of AGA and SGA is positively correlated with birth weight, indicating that HO-1 activity in cord blood is related to intrauterine growth and neonatal nutritional status.