论文部分内容阅读
目的用人血清清蛋白免疫损伤Ｗｉｓｔａｒ大鼠造成肝纤维化模型，观察中药抑肝纤和促肝细胞生长素对大鼠肝纤维化的阻断作用．方法将不同浓度（含生药１２ｋｇ／Ｌ或２４ｋｇ／Ｌ两种）中药抑肝纤（主要含黄芪，丹参，鳖甲，牡蛎等）和促肝细胞生长素用于治疗组并与模型组和对照组进行对比，综合分析血清肝纤维化指标（ＰＣⅢ，ＨＡ，ＬＮ）、肝组织病理改变、肝组织胶原纤维和网状纤维病理图象定量分析等结果．结果治疗组血清肝纤维化指标ＰＣⅢ（μｇ／Ｌ；１２ｋｇ／Ｌ组为９２４±２５２ｖｓ１５９６±３７０，Ｐ＜００１）ＨＡ（μｇ／Ｌ；１２ｋｇ／Ｌ组为２４３７±１０６８ｖｓ４８１２±１２１１，Ｐ＜００１）ＬＮ（μｇ／Ｌ；１２ｋｇ／Ｌ组为８８９±２６４ｖｓ１４２３±５４４，Ｐ＜００１）均低于模型组，接近正常组（三项指标分别为９１６±３２６，２３６６±１２１２及９２５±２３５）差异有显著性．光镜下肝组织病理变化，肝组织中胶原纤维和网状纤维病理图象定量分析结果治疗组优于模型组，尤以中药低浓度组更为明显（Ｐ＜００１或Ｐ＜００５）．结论研究结果表明，中药抑肝纤和促肝细胞生长素如能早期应用，可有效地阻?
OBJECTIVE To immunostaining Wistar rats with human serum albumin to induce hepatic fibrosis and to observe the inhibitory effect of traditional Chinese medicine inhibiting hepatic fibrosis and promoting hepatocyte growth factor on hepatic fibrosis in rats. Methods The different concentrations (including crude drugs 1 2kg / L or 2 4kg / L two kinds of Chinese medicine inhibit liver fiber (mainly containing Astragalus, Salvia, turtle shell, oysters, etc.) and hepatocyte growth-promoting hormone used in the treatment group and The model group and the control group were compared. The results of serum liver fibrosis index (PC Ⅲ, HA, LN), pathological changes of liver tissue, quantitative analysis of liver fibrosis and reticular fibrosis were analyzed. Results Serum fibrosis indicators of treatment group PC Ⅲ (μg / L; 1 2kg / L group was 924 ± 25 2vs159 6 ± 37 0, P <0 01) HA / L group was 243.7 ± 106.8 vs 481.2 ± 121.1, P <001) LN (μg / L; 12kg / L group was 889 ± 264vs1423 ± 544 , P <001) were lower than the model group, close to the normal group (three indicators were 91 6 32 6,236 6 ± 121 2 and 92 5 23 5) was significantly different . The pathological changes of liver tissue under light microscope, quantitative analysis of pathological images of collagen fibers and reticular fibers in liver tissue in the treatment group were better than those in the model group, especially in the low-concentration group (P <001 or P <0 05). Conclusions The results show that the anti-liver fibrosis and hepatocyte growth promoting hormone as early application, can effectively block?