论文部分内容阅读
以Fe3O4作为磁性载体包覆二氧化硅,并通过表面修饰引入碳碳双键,然后采用分子印迹技术,以孔雀石绿为模板分子,α-甲基丙烯酸和4-乙烯基吡啶作为双功能单体,乙二醇二甲基丙烯酸脂为交联剂,通过偶氮二异丁腈引发制备孔雀石绿的磁性印迹聚合物(MMIPs)并对单体和模板分子比例进行优化;分别采用红外光谱(FTIR)、振动样品磁强计(VSM)和透射电子显微镜(TEM)等仪器分析手段,对MMIPs的物理性质及结构进行表征;采用平衡吸附、动力学吸附和选择性吸附实验考察了材料对孔雀石绿的特异性识别能力;运用Scatchard分析,模拟得出磁性印迹聚合物具有两类不同的结合位点,其解离常数分别为1.469 mg/L和8.518 mg/L,对孔雀石绿的最大表观吸附量分别为2.97 mg/g和6.02 mg/g;将该材料应用在鱼肉样品中孔雀石绿及其代谢物隐色孔雀石绿的分离富集,经过对提取液的磁性固相萃取,在低浓度下(2,5,10μg/kg),孔雀石绿和隐色孔雀石绿的样品加标回收率都在合理范围内。
Silica was coated with Fe3O4 as a magnetic carrier and carbon-carbon double bond was introduced by surface modification. Then, molecular imprinting technique was used to make malachite green as template molecule, α-methacrylic acid and 4-vinylpyridine as bifunctional mono MAO and ethylene glycol dimethacrylate as crosslinkers were synthesized by azobisisobutyronitrile (MMIPs). The ratio of monomer to template was optimized. (FTIR), vibrating sample magnetometer (VSM) and transmission electron microscopy (TEM) were used to characterize the physical properties and structure of MMIPs. The equilibrium properties of the MMIPs were investigated by means of equilibrium adsorption, kinetic adsorption and selective adsorption experiments. Malachite green. According to the Scatchard analysis, two different types of binding sites of the magnetic imprinted polymers were obtained. Their dissociation constants were 1.469 mg / L and 8.518 mg / L, respectively. The maximum apparent adsorption capacity was 2.97 mg / g and 6.02 mg / g, respectively. The material was applied to the separation and enrichment of malachite green and its metabolite leucomalachite green in fish samples. After the magnetic solid phase Extract at low consistency Lower (2,5,10μg / kg), recoveries were malachite green and malachite green leuco are within a reasonable range.