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为解决盾叶薯蓣离体培养中试管苗移栽困难的难题,以盾叶薯蓣带腋芽的茎段为外植体,借助正交试验设计方法,离体诱导出类原球茎并建立了类原球茎微繁殖技术体系。结果表明:以带腋芽茎段为外植体诱导致密愈伤组织的适宜培养基为MS+6-BA2.0mg/L+NAA0.4mg/L+KT0.6mg/L+蔗糖3%;类原球茎诱导和增殖培养基为:MS+6-BA4.0mg/L+KT1.0mg/L+蔗糖6%;类原球茎生根培养基:1/2MS+NAA 0.3mg/L+IAA 0.8mg/L+活性炭0.3%+蔗糖1.5%。经该途径诱导得到的生根类原球茎植株经炼苗后移栽的成活率可达到90%以上。
In order to solve the difficult problem of in vitro culture of Dioscorea zingiberensis in vitro culture, the stem segments of Dioscorea zingiberensis with axillary buds were used as explants. By means of orthogonal experimental design, protocorm-like bodies were induced and proto-norms Bulb micropropagation technology system. The results showed that the appropriate media for inducing the dense callus with axillary buds as explants was MS + 6-BA 2.0 mg / L + NAA 0.4 mg / L + KT 0.6 mg / L + sucrose 3% The medium for induction and proliferation was: MS + 6-BA 4.0 mg / L + KT 1.0 mg / L + sucrose 6%; Protocorm-like rooting medium: 1/2 MS + NAA 0.3 mg / L + IAA 0.8 mg / L + % + Sucrose 1.5%. The root-like protocorm-like plants induced by this pathway could survive more than 90% after being harvested.