目的了解保洁员对感染知识的掌握情况。方法 2012年3月-5月结合《医院感染管理办法》和《消毒技术规范》的部分章节及相关参考文献,采用自行设计的调查表,对52名保洁员的职业防护知识进行不记名问卷调查。结果保洁员对感染知识缺乏,六步洗手法的知晓率仅为30.7%;摘手套后洗手的执行率为21.1%;工作中坚持戴口罩者仅占28.9%。结论保洁员在日常工作中存在较为严重的医院感染隐患,应采取适合的培训方法,加大对保洁员的培训力度,加强对其工作过程的监督与管理,提高工作质量,有效控制医院感染。 Objective To understand the cleaner’s knowledge of infection mastery. Methods From March to May of 2012, based on some chapters of “Management Rules of Nosocomial Infection” and “Technical Specifications of Disinfection” and relevant references, a self-designed questionnaire was used to conduct an anonymous questionnaire survey on occupational protection knowledge of 52 cleaners . Results Cleaners lack of knowledge of infection, six-step wash method awareness was only 30.7%; hand-seated after the implementation of the glove was 21.1%; insisted wearing a mask only 28.9% of the work. Conclusion The cleaners have more serious hidden dangers of hospital infection in daily work. Appropriate training methods should be adopted to increase the training of the cleaners, to strengthen the supervision and management of the work process, to improve the quality of work and to effectively control the nosocomial infection.