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目的观察和研究人工合成家蝇抗真菌肽MAF-1A对白色念珠菌致病性的影响。方法以白色念珠菌ATCC10231为效应菌,检测MAF-1A在MIC浓度下不同时间白色念珠菌的孢子萌发率及芽管形成率,瑞氏染色后显微镜计数,观察MAF-1A预处理后及干预下,酵母相和菌丝相白色念珠菌定植人口腔黏膜上皮细胞能力变化;分别设立氟康唑(fluconazole,FLC)和PBS为阳性对照和阴性对照。结果 MAF-1A作用白色念珠菌后,孢子萌发率及芽管形成率小于阳性对照和阴性对照组(P<0.05);MAF-1A可减少白色念珠菌酵母相细胞或菌丝相细胞对人口腔黏膜上皮细胞的粘附数,且减少酵母相细胞粘附作用强于菌丝相细胞(P<0.05);MAF-1A预处理白色念珠菌后,对人口腔黏膜上皮细胞粘附数少于MAF-1A干预组的细胞粘附数(P<0.05)。结论 MAF-1A可通过抑制白色念珠菌孢子萌发和芽管的形成以及有效降低白色念珠菌对人口腔黏膜上皮细胞粘附能力,从而影响白色念珠菌的致病能力。
Objective To observe and study the effects of artificially synthesized antifungal peptide MAF-1A on the pathogenicity of Candida albicans. Methods Candida albicans ATCC10231 was used as an effector to detect the spore germination rate and germ tube formation rate of Candida albicans at different concentrations of MAF-1A at different MIC concentrations. After Wright’s stain, the number of microspheres was observed. After MAF-1A pretreatment and intervention , Yeast phase and mycobacterial Candida albicans colonization of human oral mucosal epithelial cells were changed; fluconazole (fluconazole, FLC) and PBS were positive control and negative control. Results After MAF-1A-treated Candida albicans, the spore germination rate and the rate of germ tube formation were less than those of the positive and negative control groups (P <0.05). MAF-1A could reduce the effect of Candida albicans yeast or mycelial cells on human oral cavity Mucosal epithelial cells adhesion, and reduce the yeast phase cell adhesion stronger than mycelial cells (P <0.05); MAF-1A pretreatment Candida albicans, the adhesion of human oral epithelial cells less than MAF -1A intervention group cell adhesion (P <0.05). Conclusion MAF-1A can affect the pathogenicity of Candida albicans by inhibiting the spore germination and germ tube formation of Candida albicans and reducing the adhesion of Candida albicans to human oral epithelial cells.