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目的探讨粒细胞集落刺激因子(G-CSF)对严重免疫缺陷(SCID)小鼠急性脑梗死的治疗作用。方法SCID小鼠大脑中动脉闭塞后,立即皮下注射G-CSF,连续3d,对照组注射同样剂量磷酸缓冲液(PBS),3d后观察脑梗死体积变化、梗死边缘血管再生情况、细胞凋亡和炎症反应情况。结果G-CSF对SCID小鼠急性脑梗死后梗死体积无影响,能促进脑梗死边缘血管再生和抑制细胞凋亡,但梗死边缘CD11b和F4/80阳性细胞数均较对照组明显增加,差异均有统计学意义(均P<0.05)。结论SCID小鼠急性脑梗死后,立即注射G-CSF对脑组织有保护作用,但同时也加重急性梗死后的炎症反应。
Objective To investigate the therapeutic effect of granulocyte-colony stimulating factor (G-CSF) on acute cerebral infarction in severe immunodeficient (SCID) mice. Methods SCID mice were injected with G-CSF subcutaneously immediately after MCA occlusion. The control group was injected with the same dose of phosphate buffered saline (PBS) for 3 days. The changes of infarct volume, infarction revascularization, apoptosis and Inflammatory reaction. Results G-CSF had no effect on the volume of infarction after acute cerebral infarction in SCID mice, which could promote the angiogenesis and inhibit the apoptosis of the infarcts. However, the numbers of CD11b and F4 / 80 positive cells in infarct border were significantly higher than those in control group There was statistical significance (all P <0.05). Conclusion Immediate injection of G-CSF into SCID mice after cerebral infarction has a protective effect on brain tissue, but it also aggravates the inflammatory response after acute infarction.