论文部分内容阅读
目的：观察选择性阿片δ受体拮抗剂纳曲酮（ｎａｌｔｒｅｘｏｎｅ）对移植排异反应的影响。方法：整体实验以小鼠异体异位心肌组织移植为模型，ｉｐｎａｌｔｒｉｎｄｏｌｅ或纳曲酮（５ｍｇ·ｋｇ－１，ｂｉｄ），观察移植心肌组织的存活时间；离体实验中，将两种不同品系小鼠淋巴细胞混合培养，观察ｎａｌｔｒｉｎｄｏｌｅ、纳曲酮或亮脑啡肽对淋巴细胞反应的影响。结果：Ｎａｌｔｒｉｎｄｏｌｅ可显著延长小鼠异体异位移植心肌组织的存活时间；并明显抑制混合淋巴细胞反应。Ｎａｌｔｒｉｎｄｏｌｅ的上述作用均较纳曲酮强。阿片δ受体激动剂亮脑啡肽（ＤＳｅｒ２Ｌｕｅｅｎｋｅｐｈａｌｉｎ）可以促进混合淋巴细胞反应。结论：阿片δ受体在移植免疫排异反应中起重要介导作用，ｎａｌｔｒｉｎｄｏｌｅ对此移植排异反应有明显抑制作用。
Objective: To observe the effect of naltrexone, a selective opioid receptor antagonist, on the rejection of transplantation. Methods: The whole experiment was performed in mice model of allogeneic heterotopic myocardial transplantation, ipnaltrindole or naltrexone (5mg · kg-1, bid) to observe the survival time of the transplanted myocardium. In vitro experiments, two different strains Mouse lymphocytes were mixed culture to observe the naltrindole, naltrexone or enkephalin on lymphocyte reaction. Results: Naltrindole significantly prolonged the survival time of allogeneic xenotransplanted myocardium in mice and significantly inhibited the mixed lymphocyte reaction. The above effects of Naltrindole are stronger than naltrexone. Opioid δ agonist enkephalin (D Ser-Lue enkephalin) can promote mixed lymphocyte reaction. CONCLUSION: Opioid δ receptor plays an important mediating role in the rejection of transplanted immunity. Naltrindole significantly inhibits this rejection.