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本文采用出生２４ｈ以内的Ｗｉｓｔａｒ大鼠皮层神经细胞进行体外原代培养。观察了天门冬氨酸［Ａｓｐ：内源性诱导一氧化氮（ＮＯ）生成剂］对神经细胞的毒性作用，并用ＮＯ合成酶（ＮＯＳ）抑制剂：Ｎ─硝基─Ｌ─精氨酸（ＮＮＬＡ）和尼莫地平进行干预试验，通过观察神经细胞的形态学改变，发现ＮＯ对于体外培养神经细胞的毒性作用与动物试验结果基本一致；Ａｓｐ介导内源性ＮＯ合成增加可能是其产生神经细胞毒性的主要途径之一；调控ＮＯ生成量或阻断其合成通路，具有保护神经细胞免遭兴奋性氨基酸（ＥＡＡ）毒性的作用。
In this paper, Wistar rat cortical neurons born within 24 hours of in vitro primary culture. Aspartate [Asp: an endogenous induced nitric oxide (NO) -producing agent] was tested for its toxic effects on neurons and treated with NO synthase (NOS) inhibitor N-nitro-L-arginine NNLA) and nimodipine. Through observing the morphological changes of nerve cells, it was found that the toxic effect of NO on cultured nerve cells in vitro was consistent with that of animal experiments. Asp-mediated increase of endogenous NO synthesis may be the result of its production of nerve Cytotoxicity of one of the main ways; regulating NO production or blocking its synthetic pathway, with the protection of nerve cells from excitatory amino acids (EAA) toxicity.