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目的研究不同给药剂量注射用丹参多酚酸中迷迭香酸、丹酚酸B在大鼠体内的药代动力学特征。方法注射用丹参多酚酸以低(10 mg/kg)、中(25 mg/kg)、高(60 mg/kg)剂量以及累积剂量(25 mg/kg,连续给药7天)对大鼠进行静脉注射给药,以氯霉素作为内标物,采用UPLC-DAD-MS/MS分析手段检测血浆中迷迭香酸、丹酚酸B的浓度,绘制药物浓度-时间曲线并计算药代动力学参数。结果不同剂量注射用丹参多酚酸给药后,迷迭香酸和丹酚酸B的药代动力学行为均符合开放性二室模型一级动力学过程。结论该方法准确度高,专属性强,分析速度快,适用于注射用丹参总酚酸中迷迭香酸、丹酚酸B在大鼠血浆中的药代动力学分析。研究结果可为注射用丹参多酚酸的体内过程研究及其临床合理使用提供参考。
Objective To study the pharmacokinetics of rosmarinic acid and salvianolic acid B in salvia miltiorrhiza polyphenols for different dosages. Methods Salviae miltiorrhizae polyphenols were injected intraperitoneally on rats (10 mg / kg, 25 mg / kg, 60 mg / kg) and cumulative dose (25 mg / Intravenous injection, taking chloramphenicol as internal standard, the concentration of rosmarinic acid and salvianolic acid B in plasma was detected by UPLC-DAD-MS / MS, and the drug concentration-time curve was drawn and calculated Kinetic parameters. Results Pharmacokinetics of rosmarinic acid and salvianolic acid B were consistent with the first-order kinetic model of open two-compartment model after Salviae miltiorrhiza polyphenols were administered at different doses. Conclusion The method is accurate, specific and rapid in analysis. It is suitable for pharmacokinetic analysis of rosmarinic acid and salvianolic acid B in plasma of rats. The results of this study may provide reference for the study of in vivo process of Salvia miltiorrhiza polyphenols for injection and its clinical rational use.