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目的:研究血府逐瘀汤对D-氨基半乳糖氨(D-GalN)所致小鼠肝损伤的保护作用。方法:NIH小鼠50只,随机分5组,分别为正常对照组,模型组,血府逐瘀汤高剂量组、低剂量组和齐墩果酸片治疗组;血府逐瘀汤高、低剂量组和齐墩果酸片组小鼠分别灌胃给药,每天1次,连续4d,末次给药1h后,除正常对照组外,其余各组小鼠均腹腔注射D-GalN800mg/Kg,24小时后采血,检测血清ALT活性、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并观察肝组织病理学变化。结果:血府逐瘀汤高剂量组血清ALT活性和MDA含量均明显低于模型组(P<0.01)、小鼠血清SOD的活性明显高于模型组(P<0.01);与齐墩果酸片组比较,血府逐瘀汤高剂量组ALT、SOD活性和MDA含量差异均无显著性意义(P>0.05);血府逐瘀汤高剂量组肝组织病理改变明显减轻。结论:血府逐瘀汤对D-GalN所致小鼠肝损伤具有较好的保护作用,其保肝机制与抗脂质过氧化损伤密切相关。
Objective: To investigate the protective effect of Xuefu Zhuyu Decoction on liver injury induced by D-galactosamine (D-GalN) in mice. METHODS: Fifty NIH mice were randomly divided into 5 groups: normal control group, model group, Xuefu Zhuyu Decoction high-dose group, low-dose group and oleanolic acid tablet treatment group; Xuefu Zhuyu Decoction; The mice in the low-dose group and the oleanolic acid tablet group were intragastrically administered once a day for 4 days, and after the last administration for 1 hour, except for the normal control group, the mice in each group were intraperitoneally injected with D-GalN 800 mg/Kg. After 24 hours, blood was collected and serum ALT activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured, and the pathological changes of the liver were observed. Results: Serum ALT activity and MDA content were significantly lower in Xuefu Zhuyu Decoction group than in the model group (P<0.01), and serum SOD activity in mice was significantly higher than that in the model group (P<0.01); and oleanolic acid Compared with the tablet group, there was no significant difference in ALT, SOD activity and MDA content in the high dose group of Xuefu Zhuyu Decoction (P>0.05). The pathological changes of liver tissue in the Xuefu Zhuyu Decoction high dose group were significantly reduced. Conclusion: Xuefu Zhuyu Decoction has a good protective effect on liver injury induced by D-GalN in mice, and its mechanism of hepatoprotection is closely related to anti-lipid peroxidation injury.