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目的了解香港海鸥形菌(LH)对理化生因子的抵抗力。方法用紫外线、高温、干燥、pH、常用多种化学消毒剂及部分抗菌药物处理两株分离于病人的香港海鸥形菌,定量观察其生长情况。结果温度≥56℃即可灭活香港海鸥形菌;紫外线照射30s杀灭率>90%,照射20min可完全灭菌;菌株在pH>8.5或<5.0条件下不能存活,最适生存pH范围为5.5～8.5;在3%以上盐浓度的肉汤中不生长;对化学消毒剂和干燥较敏感,除对2%碳酸氢钠、2%来苏儿、0.1%迭氮钠和70%乙醇有一定耐受力外,其余消毒剂均可在30s内杀灭该菌;香港海鸥形菌在自然干燥后15min即全部死亡;在18种常见抗菌药物中,仅对头孢噻吩、头孢哌酮、氨苄西林、头孢唑啉、头孢他啶、克林霉素耐药,其余均敏感。结论香港海鸥形菌对多数理化生因子抵抗力较弱,常用的消毒剂能够有效杀灭LH。
Objective To understand the resistance of Physiotherapeutic factors to Hyoscyamura (LH) in Hong Kong. Methods Two strains of Hong Kong seagull isolated from the patients were treated with ultraviolet ray, high temperature, dryness, pH, and several commonly used chemical disinfectants and some antibacterial drugs to quantitatively observe their growth. Results The temperature of ≥56 ℃ could inactivate the genitalia of Hong Kong. The killing rate was> 90% at 30 s after UV irradiation and was completely sterilized at 20 min. The strain could not survive at pH> 8.5 or <5.0. The optimal pH range was 5.5 to 8.5; does not grow in broth at salt concentrations above 3%; is sensitive to chemical disinfectants and to dryness, except for 2% sodium bicarbonate, 2% laurel, 0.1% sodium azide and 70% ethanol Some of the other disinfectants could kill the bacteria within 30s; all of them died after 15min of natural drying; Among the 18 kinds of common antibacterials, they were only able to kill cefalotin, cefoperazone, Xilin, cefazolin, ceftazidime, clindamycin resistance, the rest are sensitive. Conclusion The results showed that H. guppy was less resistant to most physico-chemical factors and commonly used disinfectants could effectively kill LH.