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目的观察选择性环氧合酶2抑制剂塞来昔布体外诱导胃癌SGC-7901细胞凋亡的作用及其机制。方法用吖啶橙/溴化乙啶染色结合荧光显微镜、流式细胞仪(FCM)技术观察不同浓度的塞来昔布对胃癌SGC-7901细胞凋亡的影响,用流式细胞仪技术检测Fas和FasL蛋白表达。结果荧光染色法显示塞来昔布在15～120μmol/L时诱导胃癌SGC-7901细胞凋亡,且呈浓度和时间依赖性。FCM显示不同浓度的塞来昔布作用SGC-7901细胞48h后,凋亡率分别为8.02%～50.81%,呈浓度依赖性。塞来昔布上调胃癌SGC-7901细胞Fas蛋白的表达,下调FasL蛋白的表达。结论塞来昔布可诱导胃癌SGC-7901细胞凋亡;Fas/FasL表达的改变可能是塞来昔布诱导胃癌SGC-7901细胞凋亡的机制之一。
Objective To observe the effect of selective cyclooxygenase 2 inhibitor celecoxib on inducing gastric cancer SGC-7901 cells apoptosis and its mechanism. Methods The apoptosis of gastric cancer cell line SGC-7901 was observed by acridine orange / ethidium bromide staining combined with fluorescence microscopy and flow cytometry (FCM). Flow cytometry was used to detect Fas And FasL protein expression. Results Fluorescence staining showed that celecoxib could induce apoptosis of gastric cancer SGC-7901 cells in a concentration-dependent and time-dependent manner at 15 ~ 120μmol / L. FCM showed that the apoptosis rates of SGC-7901 cells treated with different concentrations of celecoxib for 48 h were 8.02% ~ 50.81%, respectively, in a concentration-dependent manner. Celecoxib up-regulated the expression of Fas protein in gastric cancer SGC-7901 cells and down-regulated the expression of FasL protein. Conclusion Celecoxib can induce apoptosis of gastric cancer SGC-7901 cells. The change of Fas / FasL expression may be one of the mechanisms of celecoxib inducing apoptosis of gastric cancer SGC-7901 cells.