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一般果树植物的种子,均需经过一个相当长的后熟休眠阶段和低温处理过程,才能在第二年春天播种后进行正常的萌发和生长。通过试验,我们可以随时用一定浓度的吲哚乙酸(IAA)处理沉睡着的果树种子,诱导它们进入正常的萌发和生长。一、试材与方法 (一)、用去皮的苹果杂交种子或一般苹果种子,先在70％的酒精溶液里消毒1～1.5分钟,再在的0.1％汞溶液里消毒5～6分钟,然后用蒸溜水冲洗三遍,最后在无菌条件下,将其接种附在加0.5ppm六苄基嘌呤和1～1.5ppm吲哚乙酸的MS培养基上。然后将其移于室温26～27℃,相对湿度80％左右和光照1000～1500勒克斯条件下培养1～2周后,再转入沙培和土培。
The seeds of common fruit trees need to go through a rather long post-maturing dormancy stage and a low-temperature treatment process to be able to germinate and grow normally after sowing in spring next year. Through experiments, we can treat the sleeping fruit tree seeds with indole acetic acid (IAA) at any time to induce their normal germination and growth. First, the test material and methods (A), with peeled apple hybrid seeds or general apple seeds, sterilized in 70% alcohol solution for 1 to 1.5 minutes, and then sterilized in 0.1% mercury solution for 5 to 6 minutes, Then rinsed three times with distilled water and finally inoculated on sterile MS medium supplemented with 0.5 ppm hexabutylpurine and 1 to 1.5 ppm indole acetic acid. Then it was moved to room temperature 26 ~ 27 ℃, relative humidity of about 80% and illumination 1000 ~ 1500 lux culture for 1 to 2 weeks, then transferred to sand culture and soil culture.