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目的:研究利鲁唑(riluzole)对受损背根节(DRG)A类神经元的不同模式自发放电的作用,并探讨其可能机制。方法:本实验在大鼠L5椎间孔置入不锈钢柱制备大鼠DRG慢性压迫模型(chronic compression of DRG,CCD)上,利用在体单纤维记录的方法观察DRG局部浸浴利鲁唑(500μmol/L)对受损DRG神经元自发放电的影响。结果:(1)CCD大鼠手术后表现出明显的损伤侧机械刺激缩足阈值降低(P<0.05);(2)利鲁唑对受损DRG神经元自发放电具有明显抑制作用(n=30,P<0.05);(3)利鲁唑对非周期放电频率的抑制率显著大于对周期放电频率的抑制率(P<0.05)。结论:利鲁唑对慢性压迫DRGA类神经元的自发放电有显著抑制作用,对非周期放电的抑制作用明显大于对周期放电的作用,呈现出“非周期敏感”的特点,提示受损DRG神经元非周期自发放电行为处于更加不稳定的动力学状态。
Objective: To investigate the effect of riluzole on spontaneous firing of different types of DRG neurons and to explore its possible mechanism. Methods: In this study, we observed the effects of DRG local immersion riluzole (500μmol / L) on chronic compression of DRG (CCD) prepared by placing L5 intervertebral foramen into rats’ / L) on spontaneous discharge in injured DRG neurons. (2) Riluzole significantly inhibited the spontaneous discharge of injured DRG neurons (n = 30) (P <0.05); (2) , P <0.05). (3) The inhibitory rate of riluzole to non-periodic discharge was significantly higher than that of periodic discharge (P <0.05). Conclusion: Riluzole significantly inhibits the spontaneous discharge of DRGA-like neurons in chronic compression. The inhibitory effect of riluzole on the non-periodic discharge is significantly greater than that on the periodic discharge, showing the characteristic of “non-periodic sensitivity” DRG neuronal aperiodic spontaneous discharge behavior in a more unstable kinetic state.