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建立了乳珍中纳他霉素的高效液相色谱测定方法，原国标方法1GB／T21915-2008中，测定乳珍的回收率不到80％，并对该方法进行了改进，改进后回收率在95％以上，检出限在0．5mg／kg,线性范围0．5～20μg／ml。该方法操作简单，重现性好。“,”A method for HPLC determination of natamycin in bovine colostrum powder ,for the GB Code Method ,coefficient recovery no more then 80%.The coefficient recovery.is more then 95%,when it＇ s improved .The limit of determination for the method is 0.50.5mg/kg, and the linear programming is about 0.5-20 μg/ml. This method is very simply for determination, the return for determined result is good.