目的对泰国产姜科姜黄属植物多叶姜黄(Curcuma comosaRoxb.)干燥根茎甲醇提取物的化学成分进行分离和结构鉴定。方法C.comosaRoxb.的甲醇提取物经乙酸乙酯-正丁醇-水依次萃取后,对各萃取部分采用正-反相硅胶柱色谱和制备型HPLC进行分离,经理化常数测定、核磁共振技术分析等方法鉴定了化合物的结构。结果分离得到8个化合物,分别被鉴定为异蓬莪术环二烯酮(isofuranodienone,1),蓬莪术环二烯酮(furanodienone,2),1(10)Z,4Z-蓬莪术二烯-6-酮[1(10)Z,4Z-furanodiene-6-one,3],泽泻醇(alismol,4),2,2,6-三甲基-1-氧螺[2,5]辛-5-烯-4-醇(2,2,6-trimethyl-1-oxaspiro[2,5]oct-5-en-4-ol,5),1-羟基-α,α,4-三甲基-3-环己烯-1-甲醇(1-hydroxy-α,α,4-trimethyl-3-cyclohexene-1-methanol,6),6-羟基-3(1-羟基-1-甲基乙基)-6-甲基-2-环己烯-1-酮(6-hydroxy-3(1-hydroxy-1-methylethyl)-6-methyl-2-cyclo-hexen-1-one,7),(1S,2S,4R)-1,8桉叶素-2-O-β-D-葡萄糖苷((1S,2S,4R)-2-hydroxy-1,8-cineoleβ-D-glucopyranoside,8)。结论化合物3~8为首次从该属植物中分离得到。 OBJECTIVE: To isolate and identify the chemical constituents from the methanol extract of dry rhizome of Curcuma comosa Roxb. Methods The methanol extracts of C.comosa Roxb. were extracted with ethyl acetate-n-butanol-water, and the extraction fractions were separated by positive-phase reversed-phase silica gel column chromatography and preparative HPLC. The determination of the managerial constants and NMR techniques were performed. Methods such as analysis identified the structure of the compound. Results Eight compounds were isolated and identified as isofuranodienone (1), furanodienone (2), 1 (10) Z, and 4Z-carrageenenene diene-6, respectively. -keto[1(10)Z,4Z-furanodiene-6-one,3], alismol (4), 2,2,6-trimethyl-1-oxospiro[2,5]- 5-en-4-ol (2,2,6-trimethyl-1-oxaspiro[2,5]oct-5-en-4-ol,5), 1-hydroxy-α, α,4-trimethyl 3-Hyclohexene-1-methanol (1-hydroxy-α,α,4-trimethyl-3-cyclohexene-1-methanol,6),6-Hydroxy-3(1-hydroxy-1-methylethyl) )-6-methyl-2-cyclohexene-1-one (6-hydroxy-3(1-hydroxy-1-methylethyl)-6-methyl-2-cyclo-hexen-1-one, 7), ( 1S,2S,4R)-1,8-eosen-2-O-β-D-glucoside ((1S,2S,4R)-2-hydroxy-1,8-cineoleβ-D-glucopyranoside, 8). Conclusion Compounds 3-8 were isolated from this genus for the first time.