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从冬青卫矛内生放线菌Streptomyces flavofuscus G1的发酵液中分离得到化合物G13和G19。经核磁共振波谱及质谱分析,并结合相关文献,两个化合物分别被鉴定为phencomycin(G13)和4’-deacetyl-(-)-griseusin A(G19)。抑菌活性测定结果表明:G19对水稻白叶枯病菌Xanthomonas oryzae pv.oryzae、铜绿假单孢菌Pseudomonas aeruginosa(1.203 1)、蜡状芽孢杆菌Bacillus cereus(1.184 6)及金黄色葡萄球菌Staphyloccocus aureus(1.008 9)等供试病原细菌具有强烈的抑制作用,其最低抑菌浓度(MIC)值均为1.56μg/m L;对苹果树腐烂病菌Valsa mali和小麦赤霉病菌Fusarium graminearum菌丝生长亦有明显的抑制作用,其IC50值分别为14.70和24.35μg/m L。而G13对植物病原真菌油菜菌核病菌Sclerotinia sclerotiorum和苹果树腐烂病菌V.mali菌丝生长有强烈的抑制作用,其IC50值分别为5.21和4.82μg/m L。
Compounds G13 and G19 were isolated from the fermentation broth of Streptomyces flavofuscus G1. The two compounds were identified as phencomycin (G13) and 4’-deacetyl - (-) - griseusin A (G19) by NMR and MS, respectively. The antibacterial activities of G19 against Xanthomonas oryzae pv.oryzae, Pseudomonas aeruginosa (1.203 1), Bacillus cereus (1.184 6) and Staphyloccocus aureus 1.008 9) for the test of pathogenic bacteria has a strong inhibitory effect, the minimum inhibitory concentration (MIC) values were 1.56μg / m L; on apple tree rot Valsa mali and wheat Fusarium graminearum mycelial growth also Significant inhibition, the IC50 values were 14.70 and 24.35μg / m L. G13 had a strong inhibitory effect on the growth of plant pathogenic fungi Sclerotinia sclerotiorum and V.mali mycelia, with IC50 values of 5.21 and 4.82μg / m L. respectively.