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目的:观察姜黄素对白血病细胞株K562细胞拓扑异构酶TopoI表达的影响。方法:流式细胞术及RT-PCR法分析姜黄素作用K562细胞后TopoⅠ蛋白及mRNA含量的变化。结果:TopoI在K562细胞中高表达,TopoI蛋白的平均荧光强度为84.31±4.26,显著高于阴性对照组3.82±0.17(P<0.01);流式细胞术及RT-PCR法均提示姜黄素能显著抑制TopoⅠ的表达,25μmol/L姜黄素能抑制K562细胞TopoI蛋白的表达超过一半以上,并且随着剂量的增加,姜黄素的抑制作用逐步增强。结论:姜黄素可通过抑制TopoI的表达来抑制K562细胞的增殖,是一种新的TopoI抑制剂,具有良好的临床应用前景。
Objective: To observe the effect of curcumin on the expression of topoisomerase TopoI in K562 leukemia cell line. Methods: The changes of Topo I protein and mRNA after curcumin treatment of K562 cells were analyzed by flow cytometry and RT-PCR. RESULTS: TopoI was highly expressed in K562 cells. The average fluorescence intensity of TopoI protein was 84.31 ± 4.26, significantly higher than that of the negative control group (3.82 ± 0.17, P <0.01). Flow cytometry and RT-PCR indicated that curcumin was significantly Inhibition of Topo I expression, 25μmol / L curcumin inhibited TopoI protein expression in K562 cells more than half, and as the dose increased, the inhibition of curcumin gradually increased. Conclusion: Curcumin can inhibit the proliferation of K562 cells by inhibiting the expression of TopoI, and is a new TopoI inhibitor with good clinical application prospects.