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目的旨在研究四氧化三铁纳米颗粒在大鼠脑积水模型中的磁共振及CT成像中的应用,为临床应用提供理论依据,为合理开发该纳米颗粒奠定实验基础。方法采用单纯随机分组法将60只分为空白对照组(20只,给予无菌生理盐水注入枕大池)和实验组(40只,利用高岭土制作大鼠脑积水模型,利用MR筛选大鼠脑积水模型,向模型蛛网膜下腔内注入四氧化三铁纳米颗粒,1h后行CT检查,并于1个月后在显微镜下观察空白对照组与实验组四氧化三铁纳米颗粒在脑、心、肝、脾肺、肾等器官蓄积情况。结果 (1)实验组手术和麻醉导致6只大鼠死亡,实验组死亡率15%。对照组死亡率10%。总死亡率为13.3%。(2)实验组32只大鼠形成脑积水,在存活大鼠中模型成功率为88.9%,模型总制作成功率80.0%。(3)四氧化三铁在大鼠椎管磁共振成像中显示欠佳,CT具有较好的对比效果;(4)蛛网膜下腔内注入四氧化三铁纳米颗粒1个月后在脑、肝、脾、肺、肾等器官无明显蓄积。结论椎管内注入超磁性四氧化三铁纳米颗粒是安全可靠的,可以作为脑积水颅脑CT检查的造影剂。
The aim of this study was to investigate the application of ferroferric oxide nanoparticles in magnetic resonance imaging and CT imaging of rat hydrocephalus model and to provide a theoretical basis for clinical application and lay the experimental foundation for the rational development of this nanoparticle. Methods Sixty rabbits were randomly divided into blank control group (n = 20), sterile saline injection into occipital cisterna and experimental group (n = 40). Rat models of hydrocephalus were made by using kaolin. Water model, into the subarachnoid model of iron oxide nanoparticles, 1h after CT examination, and 1 month after the observation under the microscope blank control group and the experimental group of iron oxide nanoparticles in the brain, Heart, liver, spleen, kidney and other organs were collected.Results (1) Six rats died in the experimental group and in the anesthesia group, the death rate was 15% in the experimental group and 10% in the control group, the total mortality was 13.3%. (2) In the experimental group, 32 rats formed hydrocephalus. The model success rate was 88.9% in the surviving rats and the success rate of the model was 80.0%. (3) In the spinal magnetic resonance imaging of rats, (4) There was no obvious accumulation in the brain, liver, spleen, lung, kidney and other organs after 1 month of administration of Fe3O4 nanoparticles in subarachnoid space.Conclusion The spinal canal Into the super-magnetic iron oxide nanoparticles is safe and reliable, can be used as brain hydrocephalus CT examination Contrast agent.