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AIM:To construct a recombinant strain which expresses BabAof Helicobacter pylori(Hpylon)and to study the immunogenicityof BabA.METHODS:BabA_2 DNA was amplified by PCR and insertedinto the prokaryotie expression vector pET-22b(+)andexpressed in the BL21(DE3)E.coli strain.Furthermore,BabA immunogenicity was studied by animal test.RESULTS:DNA sequence analysis showed the sequenceof BabA_2 DNA was the same as the one published by GenBank.The BabA recombinant protein accounted for 34.8% of thetotal bacterial protein.The serum from Hpyloriinfectedpatients and Balb/c rniced immunized with BabA itself couldrecognize rBabA.CONCLUSION:BabA recombinant protein may be an potentialvaccine for control and treatment of Hpyloriinfection.
AIM: To construct a recombinant strain which expresses BabAof Helicobacter pylori (Hpylon) and to study the immunogenicity of BabA. METHODS: BabA_2 DNA was amplified by PCR and inserted in the prokaryotic expression vector pET-22b (+) andexpressed in the BL21 (DE3) E .coli strain. Stillrther, BabA immunogenicity was studied by animal test .RESULTS: DNA sequence analysis showed the sequence of BabA_2 DNA was the same as the one published by GenBank. BabA recombinant protein accounted for 34.8% of the total bacterial protein.The serum from Hpylori infected patients and Balb / c rniced immunized with BabA itself could be recognized rBabA.CONCLUSION: BabA recombinant protein may be potential vaccine for control and treatment of Hpylori infection.