辅酶 Ｎ Ａ Ｄ（ Ｐ） Ｈ 因其在生物体内氧化—还原过程中的重要作用而引起了广泛的兴趣．研究焦点在其还原的历程， 即辅酶分子中的一个氢是如何转移给底物的？通常采用 Ｎ Ａ Ｄ Ｈ 的模型来还原一些有结构特征的底物以揭示其还原反应机理．大体上有三种见解： （１） 一步负氢离子转移（２） 电子转移—夺氢 （３） 电子转移—质子转移—电子转移．由于对辅酶模型还原碳—碳双键化合物研究尚不多见，本实验室在这方面开展了一系列的工作，选用 ２溴１苯基亚乙基丙二腈，２溴１苯基亚乙基丙二酸二乙酯，（ Ｚ）α氰基β溴甲基肉桂酸乙酯，芴亚基丙二腈，１， １二硝基２，２二苯基乙烯，苯亚甲基丙二腈和邻溴甲基β，β二取代苯乙烯 （取代基为吸电子基） 等化合物为底物，与 Ｎ Ａ Ｄ Ｈ 模型物进行氧化—还原反应，对反应机理进行了系统的研究 Coenzyme N A D (P) H has attracted a great deal of interest because of its important role in the oxidation-reduction of organisms. The focus of the study is on the course of its reduction, ie, how does a hydrogen in a coenzyme molecule be transferred to a substrate? N A D H model is usually used to restore some of the structural features of the substrate to reveal its reduction reaction mechanism. There are basically three kinds of opinions: (1) one-step negative hydrogen ion transfer (2) electron transfer-hydrogen abstraction (3) electron transfer-proton transfer-electron transfer. As the coenzyme model reduction of carbon-carbon double bond compounds is still rare, the laboratory in this area to carry out a series of work, the choice of 2  brominated 1  phenylethylene malononitrile, 2  bromine 1-phenyl ethylene diethyl malonate, (Z)  α cyano  β  bromomethyl ethyl cinnamate, fluorene subunit malononitrile, 1, 1 dinitro  2, 2 diphenyl ethylene, benzylidene malononitrile and o-bromomethyl β, β-disubstituted styrene (the substituent is electron-withdrawing group) and other compounds as substrate, and N A D H model Oxidation - reduction reaction, the reaction mechanism of a systematic study