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目的：探讨细胞因子对ｒＩＬ２活化的脐血淋巴细胞粘附脐静脉血管内皮细胞（ＨＵＶＥＣ）的影响机理。方法：计数粘附率和ＡＰＡＡＰ法测细胞表型。结果：ｒＩＬ２活化淋巴细胞粘附ＨＵＶＥＣ的能力明显大于未刺激组，前者的粘附率是５６．５２％，后者是２４．５４％。ｒＩＬ１、２、８、ｒＴＮＦα可明显增强其粘附，而ｒＩＬ１０使粘附率明显下降至３８．１２％；ｒＩＬ１、８、ｒＴＮＦα增强ＣＤ４＋细胞的粘附，ｒＩＬ１０抑制ＣＤ４＋、ＣＤ５６＋的粘附。结论：活化淋巴细胞粘附ＨＵＶＥＣ的作用明显增强，ｒＩＬ１、２、８、１０和ｒＴＮＦα对这一过程具有重要的调节作用。
Objective: To investigate the effect of cytokines on the adhesion of rIL2 activated cord blood lymphocytes to human umbilical vein endothelial cells (HUVECs). Methods: Count adhesion rate and APAAP assay cell phenotype. Results: The ability of rIL-2-activated lymphocytes to adhere to HUVEC was significantly higher than that of unstimulated group. The adhesion rate of the former was 56.52% and the latter was 24.54%. rIL 1, 2, 8, rTNF α can significantly enhance its adhesion, and rIL 10 adhesion rate decreased significantly to 38.12%; rIL 1, rTNF α enhanced CD4 + cell adhesion, rIL 10 inhibits CD4 +, CD56 + adhesion. Conclusion: The activated lymphocyte adhesion HUVEC significantly enhanced rIL 1, 2,8,10 and rTNF α on this process has an important regulatory role.