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为明确脑微血管内皮细胞表面糖蛋白的变化，应用凝集素亲和组织化学的方法在石蜡切片上显示荆豆凝集素（ＵＥＡ－１）、麦胚凝集素（ＷＧＡ）和花生凝集素（ＰＮＡ）受体在正常和损伤大鼠脑微血管内皮细胞的表达。结果表明，正常大鼠、失血休克和失血再灌注大鼠脑微血管均无ＵＥＡ－１受体的表达，与文献报道的种属差异性一致，而ＷＧＡ和ＰＮＡ受体在正常和损伤时均主要存在于软脑膜血管内皮细胞，仅少数浅层皮质毛细血管阳性，显示了同一器官内不同区域血管的差异性。ＰＮＡ受体在正常软脑膜血管内皮细胞的表达为２５％，失血休克时增加到７５％，短暂的失血再灌注后基本恢复正常。用三种莨菪类药物（解痉灵、樟柳碱和６５４－２）防治后，除解痉灵外，ＰＮＡ受体的表达基本恢复正常。ＷＧＡ受体在正常软脑膜的表达为７５％，失血和失血再灌注后表达稍增强为１００％，解痉灵和６５４－２预防未显示明显作用，樟柳碱使其表达下降至５０％。上述结果明确显示了（１）脑软膜微血管与脑实质微血管内皮细胞表面糖蛋白存在差异，推测与血管的功能、代谢有密切关系；（２）微血管内皮细胞损伤后脑软膜微血管内皮细胞的ＰＮＡ和ＷＧＡ受体表达呈不同程度的上调；（３）部分莨菪类药物对内皮损伤时ＰＮＡ和Ｗ?
To clarify the changes of glycoproteins on the surface of brain microvascular endothelial cells, UEA-1, WGA and PNA were displayed on paraffin sections by means of lectin affinity histochemistry. Receptors in Normal and Injured Rat Brain Microvessel Endothelial Cells. The results showed that there was no expression of UEA-1 receptor in the brain microvessels of normal rats, hemorrhagic shock and reperfusion-injury rats, which was consistent with those reported in the literature, while WGA and PNA receptors were both normal and injury Exist in the leptomeningeal vascular endothelial cells, only a few positive superficial cortical capillaries, showing the same organ in different regions of the vascular differences. PNA receptor expression in normal leptomeningeal endothelial cells was 25%, hemorrhagic shock increased to 75%, transient hemorrhagic reperfusion basically returned to normal. With three kinds of drugs (antispasmodic spirit, anisodine and 654-2) prevention and treatment, in addition to spasmolytic spirit, PNA receptor expression returned to normal. The expression of WGA receptor was 75% in normal pia mater and slightly increased to 100% after hemorrhage and reperfusion. Antispasmodic and 654-2 showed no significant effect, while anisodine reduced the expression to 50%. The above results clearly show (1) differences in the surface glycoproteins between the brain membrane and brain parenchyma microvascular endothelial cells, presumably with the vascular function and metabolism; (2) the PNA of the brain pia mater microvascular endothelial cells after microvascular endothelial cell injury And WGA receptors were up-regulated to some extent; (3) Some of the drugs on endothelial injury PNA and W?